Stability of Total Cholesterol, Triglycerides in Serum, and Glucose in Plasma under Different Storage Conditions.

Abstract

Background: Ensuring the stability of biomolecules in serum and plasma is vital for the accuracy and reliability of clinical diagnostic tests, particularly in the assessment of lipid profile parameters and glucose concentration. Objective: To determine the prevalence of dyslipidaemia among women with PCOS attending Teaching Hospital Jaffna Methods: This was an analytical cross-sectional study. Convenient sampling method was used. Women diagnosed with PCOS (n=125) through ultra sound scanning were recruited based on Rotterdam criteria from Gynaecology and Subfertility clinics of teaching hospital Jaffna. Post menopausal women and Females under 18 years were excluded. Height and weight were measured by standard methods; Serum Total Cholesterol (TC), Triglyceride (TG), by enzymatic method; HDL-Cholesterol (HDL-C) by precipitation method were measured. LDL-Cholesterol (LDL-C) was calculated by Friedewald formula. The data were analyzed using SPSS version 25.0.

Results: The mean±SD age of the women was 29.02±5.46 years. Among them majority were unemployed (66.4%) and married (88.8%). The mean±SD Body Mass Index of the women was 27.75±5.69 kg/m2, which was in the obese level (≥27.5 kg/m2). Of all participants, elevated levels of TG (≥1.71 mmol/l, 12%), TC (≥5.18 mmol/L, 41.6%), LDL-C (≥2.60 mmol/L, 89.6%), and 91.2% were with low HDL-C (≤1.55 mmol/L). The most common abnormality was raised LDL coupled with low HDL (82.4%). Combinations of all four abnormal lipid parameters were observed only in 5.6% of the women while only 1.6% had all measured lipid levels at normal levels. Conclusions: The study underscores the high prevalence and varied dyslipidaemia patterns among PCOS women, with 98.4% exhibiting at least one type of dyslipidaemia pattern, particularly elevations in LDL-C and reductions in HDL-C. Further investigation into factors contributing to dyslipidaemia high incidence; customized management and awareness is needed for early detection of cardiovascular diseases in PCOS women.