Citation:"Nagaoka, F., Itoh, M., Samad, M.S., Takagi, H., Weerasooriya, M.V., Yahathugoda, T.C., Hossain, M., Moji, K. and Kimura, E. (2013). Visual detection of filaria-specific IgG4 in urine using red-colored high density latex beads. Parasitology International, 62,32-35."
Date:2012-09-06
Abstract:
The use of urine for the immunodiagnosis of lymphatic filariasis has a definite advantage: the sample collection is not invasive and thus well accepted by people. Urine-based ELISA to detect filaria-specific IgG4 has been used successfully. However, ELISA requires equipment such as a microplate reader, which is often not available in most endemic areas. We have developed a new visual immunodiagnosis that detects urinary IgG4 using red-colored latex beads (bead test). The sensitivity was 87.2% when ICT antigen test positive people were regarded as the standard (136/156), and the specificity was 97.2% with the non-endemic people in Japan and Bangladesh, and the urine ELISA negatives in Sri Lanka (1264/1300). In a prevalence study, the bead test could detect filarial infection more effectively than ICT test among young children in Sri Lanka, indicating the usefulness of the visual test in epidemiological studies.
