dc.contributor.author |
Balagalla, DN |
|
dc.contributor.author |
Wijesuriya, A |
|
dc.contributor.author |
Ranathunge, NP |
|
dc.contributor.author |
Perera, AMMS |
|
dc.date.accessioned |
2022-08-25T10:06:04Z |
|
dc.date.available |
2022-08-25T10:06:04Z |
|
dc.date.issued |
2016-09-01 |
|
dc.identifier.issn |
1391-3646 |
|
dc.identifier.uri |
http://ir.lib.ruh.ac.lk/xmlui/handle/iruor/7911 |
|
dc.description.abstract |
Four combinations with two levels of each 2,4-Dichlorophenoxyacetic Acid (2,4-D) (0.2mg/L, 0.5mg/L) and Indole
Acetic Acid (IAA) (1mg/L, 4mg/L) together with 0.1 mg/L of Kinetin and 40mg/L of Cysteine Hydrochloride de termined by testing their 150 combinations for in-vitro direct shoot regeneration from sugarcane (Saccharum spp.
Hybrid) leaf tissues were further evaluated using the variety SL 96 328 in a completely randomised layout with 30
replicates to find out their near-optimal levels. Leaf spindle slices of the sugarcane variety SL 96 328 was used as
ex-plants for culture in the modified MS medium. After 3 weeks of culture, the survival of ex-plants and the num ber of shoots directly regenerated from ex-plants were recorded in one-week intervals. The concentration of
0.5mg/L of 2,4-D and 1mg/L of IAA together with 0.1mg/L of Kinetin and 40mg/L of Cysteine Hydrochloride re generated the highest number of viable shoots from leaf spindle discs after 4 weeks of culture. On average, 21
shoots were obtained from a leaf spindle disc of 2mm thickness and 3mm in diameter obtained from sugarcane
plants of 5- to7- month old. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Faculty of Agriculture, University of Ruhuna, Sri Lanka |
en_US |
dc.relation.ispartofseries |
TARE;2015 |
|
dc.subject |
2,4-Dichlorophenoxyacetic Acid |
en_US |
dc.subject |
Direct Shoot Regeneration |
en_US |
dc.subject |
Indole Acetic Acid |
en_US |
dc.subject |
In-vitro Culture |
en_US |
dc.subject |
Kinetin |
en_US |
dc.subject |
Sugarcane |
en_US |
dc.title |
NEAR-OPTIMAL LEVELS OF 2,4-DICHLOROPHENOXYACETIC ACID AND INDOLE ACETIC ACID FOR IN-VITRO DIRECT REGENERATION OF SHOOTS FROM SUGAR CANE (Saccharum spp. Hybrid) LEAF TISSU |
en_US |
dc.type |
Article |
en_US |