http://ir.lib.ruh.ac.lk/handle/iruor/7401 2026-05-01T21:14:46Z http://ir.lib.ruh.ac.lk/handle/iruor/18928 Knowledge and Practices on Laboratory Safety among Technical Staff of Faculty of Medicine, University of Ruhuna Wijewickrama, D.C.; Wickramaratne, K.A.C.; Udukumbura, S.J.; Patabendige, M. 2012-10-06T00:00:00Z http://ir.lib.ruh.ac.lk/handle/iruor/18927 Use of Retained Patient Samples in Verification of Precision of Full Blood Count test Results in a Private Sector Laboratory. Wickramaratne, K.A.C. 2013-01-01T00:00:00Z http://ir.lib.ruh.ac.lk/handle/iruor/18926 Evaluation of New, Non-cyanide and Simple Method for Blood Haemoglobin Estimation. Sirajul Muneera, M.Y.; Wickramaratne, K.A.C. Introduction Haemoglobin estimation is an important test. The standard Haemoglobin (Hb) estimation method is Cyanmethaemoglobin (HiCN) method. The Drabkin reagent used in HiCN method contains cyanide compounds which is hazardous for both environment and laboratory personnel. Aim To assess accuracy of Hb estimation by using distilled water as the reagent compared to the HiCN method. Materials and Method Retained 95 EDTA blood samples received at the Haematology laboratory, Teaching Hospital, Karapitiya were included in this study. The Hb value of each sample was estimated by standard HiCN method and using the new method (performed by using distilled water as the reagent). In Drabkin method 10 20 minutes are needed to develop stable Hb pigment for the estimation thus readings are taken at 20 minutes. Using new method, Hb concentration of all the samples were estimated immediately and after 20 minutes. Correlation studies and paired t test were taken to statistical analysis. Result A good correlation was observed between the cyanmethaemoglobin method and the new method. Paired t test values of immediate absorbance and absorbance at 20 minutes of the new method showed no significant difference from values obtained by standard Cyanmethaemoglobin method (immediate absorbance - P =0.819, Absorbance at 20 minutes - P =0.941). Discussion and Conclusion This study shows statistical agreement of results generated by new method against the standard. The new method doesn't require any reagent or toxic chemicals. However appropriate test validation study is needed to verify the applicability of new method for routine testing before it is recommended. 2014-01-01T00:00:00Z http://ir.lib.ruh.ac.lk/handle/iruor/18575 Angiomyomatous Hamartoma (AMH) of the Lymph Node; a Rare Cause for Isolated Lymphadenopath Rojika, K.B.; Wijesiri, T.W.; Liyanage, T.G. Introduction Angiomyomatous hamartoma is a benign vascular lesion of lymph node with unknown etiology. May represent previous inflammatory process or chronic blockade of lymphatic flow. It is characterized by replacement of the lymph node by the smooth muscle cells, varying caliber blood vessels and fibrous tissue. It commonly involves the inguinal and femoral lymph nodes and usually solitary. It is asymptomatic and rarely cause lymph edema of the lymph node. Only a few cases have been reported in the literature. Case report A 31 year old male presented with gradually enlarging right inguinal mass for three months duration. It was a gradually enlarging lump. He had no other lymph adenopathy or associated symptoms. Excision biopsy was done. The mass was firm to hard in consistency and measured 30x25x10 mm. The cut surface was homogenously whitish. Microscopy revealed a lymph node with altered architecture. The lymph node parenchyma was replaced by fibrous tissue and there were varying caliber blood vessels and proliferated smooth muscle bundles. Residual lymphoid follicles were noted at the periphery of the cortex. The lymph node capsule was thickened and no subcapsular or medullary sinuses were seen. There were no atypical cells or mitoses. Discussion Differential diagnoses for AMH include angiomyolipoma and vascular transformation of lymph node sinuses. AMH shows positivity for SMA and desmin in smooth muscle and CD 34 shows positivity for vascular endothelial cells. Angiomyolipoma shows positivity for HMB 45 in spindle cell component. However, these benign entities can be differentiated from careful microscopic assessment. Conclusion It is important to consider AMH when evaluating an isolated lymph adenopathy with vascular proliferation. 2024-01-01T00:00:00Z