dc.contributor.author |
Okada, Yukio |
|
dc.contributor.author |
Imendra, K. G. |
|
dc.contributor.author |
Miyazaki, Toshihiro |
|
dc.contributor.author |
Hotokezaka, Hitoshi |
|
dc.contributor.author |
Fujiyama, Rie |
|
dc.contributor.author |
Zeredo, Jorge L. |
|
dc.contributor.author |
Toda, Kazuo |
|
dc.date.accessioned |
2023-01-17T06:25:59Z |
|
dc.date.available |
2023-01-17T06:25:59Z |
|
dc.date.issued |
2007-07-17 |
|
dc.identifier.citation |
Okada, Y., Imendra, K.G., Miyazaki, T. et al. A Calcium-Receptor Agonist Induces Gustatory Neural Responses in Bullfrogs. Cell Mol Neurobiol 27, 771–781 (2007). https://doi.org/10.1007/s10571-007-9171-z |
en_US |
dc.identifier.issn |
0272- 4340 |
|
dc.identifier.uri |
http://ir.lib.ruh.ac.lk/xmlui/handle/iruor/10194 |
|
dc.description.abstract |
The effect of calcium-sensing receptor (CaR) agonists on frog gustatory
responses was studied using glossopharyngeal nerve recording and whole-cell patch clamp recording of isolated taste disc cells. Calcimimetic NPS R-467 dissolved in normal
saline solution elicited a large transient response in the nerve. The less active enantiomer
of the compound, NPS S-467 induced only a small neural response. The EC50 for NPS R 467 was about 20 lM. Cross-adaptation experiments were performed to examine the
effect of 30 lM NPS R-467 and 100 lM quinine on the gustatory neural response. The
magnitude of the R-467-induced response after adaptation to quinine was approximately
equal to that after adaptation to normal saline solution, indicating that the receptor site
for NPS R-467 is different from the site for quinine. NPS R-467 (100 lM) also induced an
inward current accompanied with conductance increase and large depolarization in two
(13%) of 15 rod cells, and a sustained decrease in outward current and small
depolarization in six (40%) other rod cells. NPS S-467 (100 lM) induced a sustained
decrease in outward current and depolarization in five (50%) of 10 rod cells. Another
calcimimetic cinacalcet (100 lM) induced an inward current accompanied with conduc tance increase in three (27%) of 11 rod cells. The results suggest that NPS R-467 induces
neural responses through cell responses unrelated to a resting K+ conductance decrease. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Springer Link |
en_US |
dc.subject |
Rana |
en_US |
dc.subject |
Gustatory neural response |
en_US |
dc.subject |
Ca2+-sensing receptor |
en_US |
dc.subject |
Calcimimetics |
en_US |
dc.subject |
Patch-clamp |
en_US |
dc.subject |
Taste disc cell |
en_US |
dc.title |
A Calcium-Receptor Agonist Induces Gustatory Neural Responses in Bullfrogs |
en_US |
dc.type |
Article |
en_US |