Abstract:
In vitro regeneration ability was greatly influenced by medium composition of
culture media which deliver nutrients into in vitro plant growth as well as in vivo
plant growth. In addition to inorganic salts, a carbon and energy source,
vitamins, and plant growth regulators, in case of specific needs of particular
species or tissues, other components, including organic nitrogen compounds,
organic acids, and a wide variety of complex natural extracts, can be important.
Raphanus sativus L. commonly known as „radish‟ is a popular vegetable crop
used by people all over the world for its culinary and medicinal properties.
„Beeralu‟ is a Sri Lankan radish variety which has been recommended for low
country in Sri Lanka. Therefore, variety „Beeralu‟ is needed to be subjected to
further tissue culture studies. Hence, the present study was carried out initially
to find out the effects of different organic additives on in vitro shoot
regeneration of Radish (Raphanus sativus L.) Var. Beeralu. Hypocotyl explants
of aseptic plantlets were cultured on MS basal medium (100ml) supplemented
with 2.5mg/L Benzyl Adenine (BAP) and 0.1mg/L 1-Naphthaleneacetic Acid
(NAA) and natural additives; rice flour (5g), grind potato tubers‟ juice
(100g/20ml) H2O, grind carrot juice (100g/20ml H2O), orange juice (10g/100ml
H2O), green gram (10g/100ml H2O) and control. Complete Randomized Design
(CRD) with five replicates was used for the study. After one month, the
numbers of regenerated shoots were counted and statistical analysis was carried
out using the Student Newman-Kuells Means Separation Test of SAS program
(9.1.3). Number of shoots which was gained by control treatment was 7
shoots/explant. The highest mean number of shoots (12 shoots/explant) from
Radish Var. Beeralu was observed in MS basal medium with 2.5mg/LBAP and
0.1mg/LNAA supplemented with orange juice (10g/100ml H2O). No shoots
were (0 shoots/explant) observed from medium with carrot juice but it induced
callus formation. Media with rice flour (6 shoots/explant), grind potato tubers
and green gram (1shoot/explant) inhibit the shoot regeneration.
