Abstract:
Activity guided fractionation of ethanolic extract of dried flowers of Aegle
marmelos, based on the anti-inflammatory effect was studied in the present
study. The ethanolic extract of the flowers was partitioned into ethyl acetate
and hexane, and the fractions were tested for anti-inflammatory effect using
carrageenan-induced paw oedema rat model. The ethyl acetate fraction was
separated into fraction 1, 2, 3, and 4 by column chromatography with
Sephadex G25 and tested for anti-inflammatory activity. Fraction 1 was
fractionated by silica column using ethyl acetate: dichlromethane (100:0 to
0:100) solvent system and obtained fractions a, b and c. They were tested for
anti-inflammatory activity. Fraction a, was separated into band 1, 2 and 3 by
preparative TLC with hexane: ethyl acetate (1:9) and tested for anti inflammatory effect. Phytochemical screening was done on fractions and the
FTIR and NMR spectra of band 3 were taken. The maximum percentage
inhibition of paw oedema (MPIPO) of ethyl acetate fraction and hexane
fraction were 82.7% and 66.1%, respectively. The MPIPO of fractions 1, 2,
3, and 4 were 86.7%, 72.3%, 75.9%, and 67.3% respectively. The MPIPO of
fractions a, b and c were 67.4%, 36.3%, and 53.1% respectively. The MPIPO
of bands 1, 2, and 3 were 45.5%, 30.9% and 53.9% respectively.The FTIR
spectrum exhibited the presence of O-H/N-H/C=O/C-Cl and C=C groups in
band 3 and the NMR readings suggested that the band 3 is required to be
purified further. Phytochemical screening revealed the presence of poly
phenols and triterpenoids in band 3and they may be the compounds that
responsible for the anti-inflammatory effect of belimal
