Abstract:
With the emergence of multidrug resistant microorganisms the world is in need of
finding new drugs. There is no published data on the antimicrobial effect of Alocasia plumbea
though this plant has been used traditionally in Ayurveda medicine for treatment of tonsillitis,
acne, snake bites, cysts and skin eruptions. Therefore the current study tested
Alocasia. plumbea to find any chemical entity with antimicrobial effect.
Shade dried and powdered tuber and aerial parts of A. plumbea were separately used
for the extraction using a solvent series of hexane, chloroform, methanol and water
respectively. Each extract was subjected to preliminary toxicity test using Brine shrimp. It was
observed that other than the water extract of the aerial and chloroform extract of the tuber, all
the other extracts did not show toxic activity. The antimicrobial activity of the extracts were
tested using both the cut well method and agar dilution method at 20mg/ml concentration.
Even though in the cut well method antimicrobial activity was not seen, in the agar dilution
method good antimicrobial activity was noted. The methanol extract of the aerial part
inhibited growth of Staphylococcus aureus, Pseudomonas aerugenosa and Escherichia coli.
The methanol extract of the tuber part and water extract of the aerial part inhibited S. aureus
and P. aerugenosa. Hexane extract of aerial and tuber inhibited only S. aureus. Chloroform
extract of aerial part inhibited Candida dubliniensis and Candida rugosa. Antimicrobial
activity with the absence of cytotoxic activity is a significant finding with reference to
Alocasia. plumbea extracts.
Therefore, the methanol and hexane extracts of Alocasia plumbea could be used for
the investigation of new antimicrobial agents. This plant is an abundant easy growing plant
and the yield of methanol extract is comparatively high, which is an advantage. As a solvent
series was used to extract, the active compounds inhibiting the growth of the microorganisms
in methanol may differ from the active compounds in other solvents with different polarities.
This gives direction for further studies to find new chemical entities with minimum toxicity to
fulfill the current need for antibiotics.
