Abstract:
The recently discovered SARS-CoV2 virus provoked a severe coronavirus
pandemic (COVID-19). Understanding the structure and function of viral
proteins is essential for comprehending viral infection and pathogenesis and
developing treatment and prevention techniques. The coronavirus nonstructural
protein 1 (nsp1) is a crucial feature with a wide range of involvement
in virus-host interactions and various sequence, structure, and functional mode
characteristics. This research aims to reveal the structural conformational
changes of nsp1 to aid in prediction by developing a homology model utilising
SWISS-Model and I-Tasser and evaluating the best model. PROCHECK,
PROSA, Errat, and Verify3D investigated and validated all predicted models
corresponding to the nsp1 homologs of SARS-CoV1, SARS-CoV2, and
MERS-CoV. SWISS-Model homology modeling for SARS-CoV2 resulted in
94.6% of builds being in the most favorable region, 5.7% in the authorised
region, 0% in the liberally permitted zone, and 0% in the denied region. The
i-Tasser model had a significantly lower percentage of builds in the most
favourable region (61.1%), higher percentages in the authorised region
(28.9%), a relatively higher percentage in the liberally permitted zone (6.0%),
and a relatively higher percentage in the denied region (4.0%). It was observed
that SARS-CoV1 nsp1 and MERS-CoV nsp1 in SWISS-Model showed higher
PROCHECK values than I-Tasser. The PROCHECK, ProSA Z-score, Errat,
and Verify3D results demonstrated that the SWISS-Model is reliable and solid
enough to be utilised in future research.
