Abstract:
Non
enzymatic glycation leads to the formation of advanced glycation end
products (AGEs) which are key mediators of the pathogenesis of chronic
diabetic complications and of accelerated skin ageing. Intermo lecular cross
linking is caused by some AGEs, affecting the function of the target
molecule. Collagen is a primary target of such AGE mediated changes. The
objective of this study was to investigate the inhibitory effects of Terminalia
arjuna (family Combr etaceae ) bark methanol extract on glycation and
glycation induced cross linking using polyacrylamide gel electrophoresis
under native and denaturing conditions. Bovine serum albumin (BSA) and
lysozyme were incubated with 0.5 M fructose for 21 days at 37°C and pH
7.4, in the presence or absence of different concentrations (0.01 1 mg/mL)
of the bark methanol extract. Appropriate controls and the standard
glycation inhibitor aminoguanidine (1 mg/mL) were used. Aliquots from
BSA and lysozyme were analyzed usi ng native polyacrylamide gel
electrophoresis (PAGE) and sodium dodecyl polyacrylamide gel
electrophoresis (SDS PAGE) respectively. Glycated BSA showed an
increase in the migration towards the anode with PAGE, when compared
with that of non glycated BSA. Hi gh molecular bands were visible with
SDS PAGE, depending on the extent of lysozyme cross linking. T. arjuna
showed inhibitory effects on glycation and glycation induced protein cross
linking at all the concentrations used from 0.01 to 1 mg/mL. In conclusio n,
methanol extract of T. arjuna bark showed strong in vitro inhibitory effects
on glycation and glycation induced protein cross linking, indicating the
value of further studies.
