Abstract:
The demand for the utilization of herbal medicine is rising due to their high
efficacy, affordability, ease of use, better adaptability with human body and lesser side
effects. Leea indica (Burm.f.)Merr. (Burulla) is a medicinal plant used in Sri Lankan
traditional medicine for treating many diseases. The present study was aimed to formulate
a novel herbal balm with Leea indica (Burm.f.) Merr. leaf extract and evaluate in vitro anti inflammatory and antioxidant activities. Two different defatted crude extracts namely,
70% aqueous acetone and 80% aqueous methanol were prepared and subjected to
preliminary phytochemical screening. Different formulations (Fb1-Fb4) were developed
by incorporating freeze dried powder of most active, defatted 70% aqueous acetone extract
and tested for their physicochemical stability parameters for period of 60 days.
Formulations were tested for in vitro anti-inflammatory activity (by Human Red Blood
Cell membrane stabilization assay) and in vitro radical scavenging activity {(by 2, 2-
diphenyl-1-picrylhydrazyl (DPPH) assay)}. The phytochemical screening of tested extracts
revealed the presence of alkaloids, flavonoids, phenols, tannins, saponins, terpenoids,
carbohydrates and phytosterols in both extracts. Among the formulated balms, Fb4 showed
the highest values of percentage inhibition (75.084 ± 0.193 % of heat induced hemolysis at
the concentration of 12.5 mg/mL) and in vitro radical scavenging activity (2.892 ± 0.008
mmol Trolox per 100 g of balm). All formulations showed no remarkable variation in
stability parameters during the period observed. It is concluded that the formulated balms
have promising in vitro anti-inflammatory and antioxidant activities and Fb4 is
recommended for further investigations of clinical trials.
