Abstract:
The present study investigated the e¡ect of supple menting feed with transgenic Synechocystis sp.
PCC6803 containing the Paralichthys olivaceus
growth hormone (GH) gene on growth, feed intake
and feed e⁄ciency ratio, muscle composition, hae matology and histology of turbot (Scophthalmus max imus L.). At the end of the 40-day feeding trial, the
speci¢c growth rate of ¢sh fed the supplemented feed
with 1.0% transgenic Synechocystis sp. PCC6803 was
21.67% higher (Po0.05) than that of control ¢sh.
Although body weight and feed e⁄ciency ratio sig ni¢cantly increased (Po0.05) in ¢sh fed the diet sup plemented with transgenic alga, feed intake and
condition factor of the experimental ¢sh were unaf fected. Muscle composition analysis showed that the
protein content was positively in£uenced by the
transgenic alga, whereas the lipid content was unaf fected. Haematological parameters, including red
blood cell, white blood cell, haemoglobin, and serum
biochemical indices, such as enzyme activities of ala nine aminotransferase, aspartate aminotransferase,
alkaline phosphatase, lactate dehydrogenase, con centrations of total protein, glucose, blood urea
nitrogen, creatinine, triglyceride and cholesterol
and ion levels of K, Na, Cl, P were not in£uenced
by supplementing the transgenic Synechocystis sp.
PCC6803. Furthermore, no histopathological altera tions were induced by transgenic alga treatment in
the stomach, intestine, liver, spleen and kidney of
the experimental ¢sh. The results of the present
study indicated that transgenic Synechocystis
sp. PCC6803 containing P. olivaceus GH gene is an ef-
¢cient growth promoter and a safe feed additive for
¢sh.
