Citation:Hasan, S.K.N., Jana, S.C., Manna, S., Dey, A., Wijesekera, K., Roy, S., Napagoda, M.T., Bag, B.G. (2020). A green route to phytofabricated synthesis of Gymnema sylvestre conjugated silver nanoparticles (GS-AgNPs) and its antimicrobial activity. Prayogik Rasayan, 4(1), 1-7. http://www.divyarasayan.org/Issues.aspx?IssueID=1013
Date:2020-01-03
Abstract:
The plant-derived chemicals present in the leaf extract of Gymnema sylvestre (LEGS) commonly known as “Gurmer” has
been utilized for the synthesis of Gymnema sylvestre conjugated silver nanoparticles (GS-AgNPs) at room temperature without any
auxiliary reducing and capping agents. Spherical shaped GS-AgNPs of 26.6 nm average diameters were observed. A selective dose
dependent antimicrobial activity of the freshly synthesized GS-AgNPs on both gram-positive Staphylococus aureus and gram negative Proteus vulgaris strain showed the same minimum inhibitory concentration (MIC) value at 50 μg/mL, as revealed by in-vitro
MIC assay. Zone inhibition study carried out by Disk agar diffusion (DAD) test showed that the GS-AgNPs is sensitive on both the
bacteria cell having a comparatively better antimicrobial activity towards Gram-negative over Gram-positive bacteria. The synthesized
GS-AgNPs showed very low toxic effect in normal human cells. Dichlorodihydrofluorescein diacetate (DCDHFDA) and ethidium
bromide-acridine orange (EB-AO) staining studies revealed that the GS-AgNPs mainly produces Reactive Oxygen Species (ROS) by
altering its electron transport chain (ETC) system and it leads to cell death or necrosis through apoptosis of bacterial cells.
