Abstract:
Binding loose compost into pellets needs different binding materials. Maintenance of pellet stability
highly depends on the binding materials used. Potable water treatment plant sludge (WTPS) has the
potential to be used as a binding material due to the high content of clay, organic matter, and
nutrients. However, compost and WTPS may contain heavy metals, toxic compounds, salts, and
growth inhibitors. Therefore, the determination of the phytotoxicity of WTPS bound compost
pellets is essential before amending them to the soil. The present study aimed to assess the
phytotoxicity of different pelleted compost using seed germination bioassay of Raphanus sativus L.
Four compost pellets were considered (T1: commercial compost pellet (100% compost), T2:
commercial integrated pellet (90% compost+10% inorganic fertilizer), T3: WTPS-bound compost
pellet (90% compost+10% WTPS), T4: WTPS-bound integrated pellet (80% compost+10%
WTPS+10% inorganic fertilizer)) and pellet aqueous extracts (PAE) were prepared, respectively.
Distilled water was used as the control. The dilution sequence of PAE as 50% and 100% were
tested for seed germination in Petri dishes in a randomized design with three replicates. pH, EC, and
selected heavy metals (Al, Zn, Cu and Cr) were determined in PAE. RSG% (Relative Seed
Germination %), RRG% (Relative radicle Growth %), and GI% (Germination Index) were
calculated for all the PAE after 72 hours. The PAE had a pH range from 6.8-7.2. The Cr was not
detected in all 100% PEA. The highest levels of Al and Zn were detected in 100% PAE of T1. All
the treatments in both 50% and 100% PAE showed GI% higher than 80% except the 100% PAE of
commercial integrated pellet (T2) and WTPS-bound integrated pellet (T4). The PAE from the T2
and T4 showed low RSG% and RRG% and thereby the GI% is low due to high EC in PAE. The
GI% of 100% PAE in T2 and T4 was 32.33% and 67.25%, respectively. The lowest values for
RSG% and RRG% were recorded for T2 as 44.44% and 72.75%, respectively. Spearman‘s Rank
Correlation coefficients were calculated for EC with RSG%, RRG%, and GI% and all variables
showed negative correlations as (-0.44), (-0.97), and (-0.69), respectively. This indicates that high
EC reduces radical growth and seed germination. PAE at 100% concentration levels of commercial
integrated pellet showed high phytotoxicity, and WTPS-bound integrated pellet showed less
phytotoxicity while other treatments did not show any sign of phytotoxicity. All the PAE at 50%
concentration level were free from phytotoxicity. Hence, 10% WTPS in w/w basis can be used as a
binding agent in pelletizing loose compost. Different WTPS concentrations should be tested in
binding process for its optimum utilization.