Abstract:
Volatilization of N H 3 into the atmosphere has become an important environmental issue
Action of urease enzyme produced by microorganism breaks down the nitrogenous
wastes (undigested proteins and: excretory uric acid) in poultry litter into N H 3 and
subsequently volatilized into the environment. Polyphonols of some plant extracts
inhibit the soil urease activity thereby reducing the release of NH3 from soil surface.
Two laboratory experiments were conducted to test whether refused tea (RT); a
polyphenol-rich by product of tea processing industry is capable of reducing N H3
emission from poultry litter. In experiment 1, broiler litter samples were incubated 1) as
it is (control; C, 2) with 3% RT extract; RT3, 3), with 3% water; W TR3,4) with 5% RT
extract; RT5 and 5) with 5% water, WTR5. In experiment 2, broiler litter samples were
incubated 1) with 0% RT powder, 2) with 3% RT powder and 3) with 5% RT powder.
In both experiments treatments were arranged according to a completely randomize
design with 5 replicates per each treatment. The volume of N H 3 emitted from each
incubation unit for 8 hours was trapped with a boric acid solution and subsequently
titrated with HC1 to determine the emission rate. Litter samples were analyzed for
moisture and pH. Emission of ammonia was expressed as mg/hour/kg of diy litter. In
experiment 1, emission of NH3 from C (1.22 mg/kg/day) reduced by 28% to 0.88
mg/hour/day when the litter sample was treated with 3% RT extract. Emission from
RT3 was around 73% of that from WTR3. Emission from RT5 was significantly lower
than that from WTR5. In experiment 2, ammonia emission rates of the litter treated
with 3% RT powder (0.72 mg/ kg/day) and with 5% RT powder (0.64 mg/kg/day) were
significantly lower than the litter which was not treated with RT powder (1.65
mg/kg/day). Mixing of RT powder at 3 or 5% with litter significantly reduced the litter
pH compared to control. It was concluded that RT could be used as a litter amendment
to reduce the emission of NH3