Abstract:
Chemical control methods have been practicing for years to control the Root Knot 
Nematodes (RKN), a devastating pest in tobacco. Due to the deleterious effects of 
chemical methods on human health and environment alternative methods need to he 
developed. With these objectives, a study was conducted to investigate biopesticidal 
approaches, including biological agents, ground neem seed and tobacco waste dust, 
for the control of RNK in tobacco. Suppression of RKN was examined under field 
condition by assessing the disease incidence with root knot count and parasitic 
nematode count. Seedling density, fresh weight, dry weight, shoot length and root 
length of tobacco also were measured to examine the growth performances. Results 
indicated that ground neem seed treatment has suppressed the RKN disease to the 
best level expressing least number of knots and parasitic nematodes. Tobacco waste 
treatment has been the second best followed by the treatment with Pseudomonas
fluorescens. Although, Trichoderma viride has not controlled the infection much it 
has significantly improved the seedling density and vegetative growth. Further 
experiments should be conducted to detect the combined effect of these 
biopesticides.