Abstract:
ScFv monoclonal antibodies were raised against Colletotrichum gloeosporioides
(Penz.) Penz. and Sacc. isolates of papaya using phage display technology. Phages
obtained after fourth round of biopanning were used to generate monoclonal
antibodies against the pathogen. Four monoclonals were identified having the
highest binding affinity to C. gloeosporioides and were selected to differentiate
isolates of C. gloeosporioides along with isolates of C. capsici, Fusarium
oxysporum f.sp. cubense and Alternaria spp. Cetyle trimethyl ammonium bromide
(CTAB) method with some modifications was followed to isolate total genomic
DNA in another experiment. Initially, DNA samples were amplified with 20
different OPA random primers and two of them were selected to amplify individual
DNA samples from the eight isolates. All monoclones found to have high binding
affinity towards different isolates of C„ gloeosporioides compared to three other
species, indicating their specificity towards C. gloeosporioides. There was a greater
variation observed among isolates according to band patterns. However, with both,
primers (OPA 3 and OPA 14), there were common bands that may be specific to C.
gloeosporioide.
