Abstract:
The effect of different substrate compositions on the growth performance of micro
propagated ornamental aquatic plant; Cryptocoryne wendtii ‘Water Trumpet’ under
submerged conditions was investigated. River sand (R), Topsoil (T), Coconut coir dust
(D) and Cattle manure (M) were used to prepare the substrates. Plastic containers (20.4
cm diameter, 19.2 cm height) were filled with one of four substrate compositions; S (R:T
1:1), C (R:D 1:1), SM (R:T 1:1 with 10% M) and CM (R:D 1:1 with 10% M). Individual
plants (9.60 ± 1.47cm and 0.13 ± 0.01g) were potted in each container and each treatment
had four replicates. Plants were cultured for 35 days under ambient temperature and light
conditions, and the water level was maintained at 10 cm above the substrate. Plant growth
parameters and environmental parameters were measured once in every two weeks. Oneway
Analysis of Variance and Duncan’s multiple range test in post-hoc analysis was
employed to test the effect of different substrates on the experimental plants. Water
temperature (26.8 ± 0.08 ℃) was not significantly different among the four treatments.
Water pH value was significantly higher in S (7.78 ± 0.32) than that in C (7.58 ± 0.26),
SM (7.69 ± 0.29), and CM (7.51 ± 0.18). Plants grown in C and SM exhibited
significantly higher total plant wet weight (0.19 ± 0.03 g and 0.23 ± 0.03 g), shoot wet
weight (0.14 ± 0.01 g and 0.17 ± 0.04 g) and the %weight gain (44.71 ± 8.94 and 77.68
± 20.32) respectively compared to other treatments. SM showed significantly the highest
total plant dry weight (19.53 ± 2.05 mg) and shoot dry weight (12.63 ± 2.15 mg)
compared to S, C and CM. It can be concluded that the application of river sand and
topsoil (1:1) with 10% cattle manure mixture (SM) is suitable for the propagation of the
ornamental aquatic plant “water trumpet” under submerged conditions.
