Abstract:
A significant number of bioactive compounds extracted from marine plants acts as free
radical scavengers. Such compounds have pharmaceutical value since many diseases are
associated with oxydative stress caused by free radicals. The present study investigated
the antioxidant properties in phytochemicals extracted from Sargassum sp. and Ulva
lactuca collected from Dikwella bay, Southern Sri Lanka. Methanolic extractions were
obtained to perform the phytochemical screening which indicated the presence of
flavonoids, terpenoids, tannins, steroids, glycosides, and saponins in both samples.
Following the extraction of phytochemicals, antioxidant activities were tested using
DPPH (2-2-diphenyl-picryl-hydrazyl) radical scavenging activity, total phenolic content
(TPC) and phosphomolybdinum assays. To determine the DPPH activity, light
absorbance was measured at 517 nm and ascorbic acid was used as the standard reference
solution. The TPC was determined by Folin-Ciocalteu colorimetric method using gallic
acid as standard reference. Phosphomolybdinum assay was conducted to evaluate the
total antioxidant activity by measuring the absorbance at 695nm. The crude
concentrations of the Sargassum sp. and Ulva lactuca were 40.20 ±7.62mg/mL and 41.76
±3.95mg/mL respectively. The DPPH radical scavenging activity of Sargassum sp., U.
lactuca and ascorbic acid showed the IC50 (half minimal inhibitory concentration) values
of 8.41±0.01mg/mL, 5.01±0.01mg/mL and 71.15±0.01mg/mL respectively. Extract of
U. lactuca gave the highest IC50 (p<0.001) value than that of ascorbic acid and Sargassum
sp. showed significantly higher TPC (106.78 ±0.36 mg GAE/g) than U. lactuca (84.75±
0.35mg GAE/g). In phosphomolybdinum assay, Sargassum sp. (113.016±1.47mg)
indicated a significantly higher antioxidant value (p<0.05) than U. lactuca (62.769±1.78
mg AAE/g). Results indicated the presence of high DPPH radical scavenging activity of
plant extracts than the standard ascorbic acid reference. Sargassum sp. exhibits the
highest DPPH radical scavenging activity, total phenolic content and total antioxidant
activity compared to the U. lactuca. Present study highlights the potential application of
U. lactuca and Sargassum sp. as possible sources of natural antioxidants in
pharmaceutical and food manufacturing industries.
