Abstract:
Intensified fish farming to meet the current demand for fish often resulted in fish
disease outbreaks. Among these diseases bacterial diseases are a major threat,
requiring early detection of the pathogens which may facilitate to undertake
suitable control measures and treatment before it may spread and reach epidertiic
status.
In this study two pathogenic bacterial species were isolated from diseased fish.
They were identified by using morphological and biochemical characters. Their
virulence were evaluated by using pathogenicity models, namely the bath challenge
and the injection challenge. Among these models, the injection challenge has been
found as the most effective way of introducing pathogens into test fish.
In the latter part of the study, efforts were made to identify a suitable primer which,
can be used as a probe for the molecular detection of the pathogen.