Abstract:
The identification of populations of Bruguiera sexangula, Bruguiera gymnorhiza and their putative hybrids in the
field is difficult using only morphological and phenological characters. Using a PCR based technique, RAPD (Random
Amplified Polymorphic DNA), the genetic variation of Bruguiera populations was studied from contrasting
climatic and geographic regions along the southwest coastal region of Sri Lanka. Out of 45 primers screened,
20 primers allowed us to observe polymorphism, not only between species (interspecific) but also within the
species (intraspecific). Analysis of RAPD data appears to be helpful in determining the genetic relationship among
populations of B. gymnorhiza and B. sexangula. RAPD markers revealed that the two species are well separated
without any hybrid position between the two taxa though they occur in mixed stands. Although sampling sizes of
populations of this study were small, genetic variation among B. gymnorhiza and B. sexangula populations could
be observed. For B. sexangula, it was possible to differentiate each of the three populations, even when using a
small number of primers.
