Evaluation of in vitro Gastroprotective Activity of an Aqueous Extract of Avipattikar Choorna Used in the Management of Gastritis (Amlapitta) .

Abstract

Background: Gastritis (Amlapitta) is a highly widespread illness in the modern world. The Avipattikar choorna (AC) is an ayurvedic polyherbal formulation which is used to treat gastritis. Objective: To evaluate in vitro gastroprotective activity of the aqueous extract of AC and formulate a novel tablet dosage form for effective and convenient usage Methods: The aqueous extract of AC was prepared according to the ayurvedic method. According to that, 60 g of AC was dissolved in 240 mL of lukewarm water, mixed well and filtered using muslin cloth. Four different concentrations were prepared using the aqueous crude extract (C1 108.58, C2-153.90, C3-253.48 and C4-615.06 mg/mL). Tablets incorporating 450 mg of dried powder of aqueous extract of AC were formulated. Three different concentrations (C5-33.33, C6 66.66, C7-100.00 mg/mL) were obtained by dissolving of formulated tablets respectively. In vitro gastroprotective activity of AC and formulated tablets was evaluated using the neutralizing effect on artificial gastric acid, neutralizing capacity using the titration method of Fordtran’s model and duration of consistent neutralization on artificial gastric acid using Vatier’s artificial stomach model. Distilled water and Eno were used as negative and positive controls, respectively. Statistical analysis was performed using One-way ANOVA. Results: All the tested concentrations of AC and formulated tablets exhibited significant (p<0.05) gastroprotective activity in comparison to negative control. Neutralizing effect of different concentrations of AC, formulated tablets and Eno showed the final pH of C1-3.25±0.00, C2 3.33±0.00, C3-3.55±0.00, C4-3.86±0.01, C5-2.04±0.02, C6- 2.36±0.01, C7-3.07±0.02, 6.31±0.01, respectively. Neutralizing capacity of different concentrations of AC and formulated tablets were C1-0.14±0.01, C2-0.19±0.01, C3-0.28±0.00, C4-3.97±0.03, C5-0.18±0.01, C6-0.36±0.01, and C7 0.52±0.01 mmol, respectively. Eno showed neutralizing capacity of 1.23±0.03 mmol. Duration of neutralization of different concentrations of AC and formulated tablets were C1-188.00±3.05, C2 206.00±1.52, C3-272.33±1.45, C4-321.00±2.07, C5-142.33±5.04, C6-194.66±3.52, and C7 267.66±5.78 sec, respectively. Eno showed the highest duration of neutralization among the tested samples (453.66±2.33 sec). Conclusion: Both AC and formulated tablets exhibited significantly higher in vitro gastroprotective activity. Stability studies are recommended for the newly formulated dosage.