Optimization of Encapsulation Efficiency of Coccinia grandis (L.) Voigt Extract Encapsulated Nanoliposomes

Abstract

Nanoliposomes are commonly used in the encapsulation and delivery of plant polyphenols. This technology offers an attractive strategy to address the limitations in bioavailability and stability of polyphenols. Leaves of Coccinia grandis (L.) Voigt is a rich source of polyphenols which exhibit antidiabetic effects. Encapsulation of C. grandis extract into nanoliposomes may lead to the development of a novel therapeutic agent against diabetes mellitus. This study attempted at optimizing encapsulation efficiency (EE) of C. grandis leaf extract encapsulated nanoliposomes at varying loading concentrations of the plant extract. Nanoliposomes were formulated by modified emulsification and ultrasonic method using ethanolic solution of phosphatidylcholine and cholesterol (20:1) as the lipid phase and phosphate buffered saline mixed with Tween® 80 (0.1% v/v) as the aqueous phase. Particle size and poly dispersity index (PDI) of the nanoparticles were determined by dynamic light scattering.