Sysnthesis and characterization of succinyl chitosan nanoparticle and study their applications in the biomedical field

Abstract

Chitosan is a deacetylated form of chitin and it is one of the most abundant naturally occurring co polysaccharides. Wound dressing is a promising medical application for chitosan and its derivatives. Chitosan is insoluble in water, which has significantly restricted its applications. Chemical modification through introduction of hydrophilic functionalities is a well known process to increase the water solubility of chitosan. The objective of this study was to synthesize N-Succinyl-Chitosan (N-Suc-Chi) nanoparticles and evaluate its ability to accelerate the wound healing process.Three different N-Suc-Chi derivatives were synthesized by changing weight ratios of succinic anhydride to chitosan as 1:1(N-Suc-Chi 1), 2:1(N-Suc-Chi 2) and 3:1(N-Suc-Chi 3).The degree of substitution (DS) was determined by a titrimetric method. The N-Suc-Chi 3 was characterized by FT-IR spectroscopy, 1H NMR spectroscopy, X-ray diffraction (XRD), zeta potential analysis, and solubility test. N-Suc-Chi nanoparticles (N-Suc-Chi NPs) were prepared with Sodium Tripolyphosphate (TPP) through ionic cross linking method. The morphology, particle size and zeta potential of the nanoparticles were investigated and shown a 58.9 ±6.2 nm particle diameter with a +38.0 ± 2.3 mV zeta potential. Further, we investigated the ability of N-Suc-Chi NPs to accelerate the wound healing process by in vitro scratch assay and WST-1 cell proliferation assay using Human Dermal Fibroblasts (HDF cell). In vitro scratch assay results revealed that N-Suc-Chi NPs has higher ability to cell migrate towards the cells free area than N-Suc-Chi treated one.The WST-1 cell proliferation assay was showing the much better proliferation and biocompatibility of N-Suc-Chi NPs.