Extraction, characterization of essential oil in leaf extract of Toddalia asiatica

Abstract

Essential oil is a mixture of low molecular weight constituents that are responsible for its characteristic aroma. They are used in several industries as raw materials such as perfumes, cosmetics, food flavor and pesticides etc. Objective of this project was to isolate and characterize the insect repellant volatile oil of Toddalia asiatica leaves. Essential oil from 40 g leaves of Toddalia asiatica was isolated by steam distillation. After the extracting of the oil qualitative tests were carried out for the detection of functional groups present. 2,4-dinitrophenyl hydrazine test revealed that the essential oil contained carbonyl functionality. In order to determine the components present in essential oil extracted, GC-MS analysis was done. Main constituent present in essential oils are trans-ethoxy-1-butene (53%), limonene (9.8%), 3-mthyl-2-butenal (4.8%), 2-metheyl-2-hexene (2.5 %), caryophylline oxide (1.3 %), bis(2-ethylhexyl) phthalate (6.4 %) and other minor components by GC yield. This results shows the essential oil of leaves of T. asiatica contained well-known insect repellant compounds.