Red mold rice water extracts to inhibit adipogenesis in vitro

Abstract

Monascus-fermented products are being used as food and medicine for over thousands of years. Due to the water solubility, adipogenesis inhibitors from Monascus-fermented products can be used in food or nutraceutical industry. This study was conducted to investigate the anti-adipogenic effects of the water extracts of Monascus pilosus NBRC4507 fermented red mold rice (RMR) using 3T3-L1 cells. RMR was prepared by cultivating M. pilosus NBRC4507 in steamed rice for 7, 10, 14, 17 and 21 days at 30 °C. The water extracts of RMR were concentrated and the resultant concentrate was freeze dried and dissolved in phosphate buffer. RMR water extracts (0.5, 0.75 and 1.0 mg/mL) were prepared using each cultivated samples at 30 °C in differentiation medium. RMR extracts on lipid accumulation inhibition in 3T3L-1 cells was measured using Oil-red O staining method.