Optimization of some fermentation conditions and composition of the fermentation medium for bio-ethanol production from poultry and cattle feed by yeast fermentation

Abstract

The study aimed to increase the bioethanol production by optimizing the fermentation conditions from poultry and cattle feed. Submerged fermentation was carried out in the presence of Baker’s yeast (Saccharomyces cerevisiae). The fermentation medium contained 50 g glucose, 2.5 g yeast extract, 1.15 g peptone, 0.25 g (NH4)2HPO4 and 0.025 g MgSO4 L-1 and inoculated (250 mL fermentation medium / 10 mL inoculum) with activated Baker’s yeast. When glucose of the fermentation medium was replaced with 50 gL-1 of different types of commercially available poultry feeds such as layer grower, chick feed, layer feed, cattle feed, rice bran, sesame oil seed cake and coconut oil seed cake separately, cattle feed (mean 0.4% v/v) and rice bran (mean 0.3% v/v) showed highest bioethanol activity compared to other carbon sources at 48 h of fermentation at 100 rpm. Glucose containing medium (control) produced a mean bioethanol percentage 3.2 (v/v) at 48 h of fermentation at 100 rpm. Cattle feed and rice bran were selected for further analysis.