Abstract:
Background: Though the blood group O donors are considered as the universal donors, their
plasma contain both types of antibodies named anti-A and anti-B. These antibodies can cause
Acute Haemolytic Transfusion Reactions (AHTR) due to passive transfer of antibodies. These
antibodies can be detected using titration techniques.
Objectives: To detect the anti-A and anti-B haemagglutinin titre in blood group O donor
plasma and find its correlation to donor age and gender.
Methods: A cross-sectional analytical study was performed with 132 blood group O donors
(44.6% females and 55.3% males). Samples were categorized into four groups according to age:
18-28, 29-38, 39-48 and 49-55 years. Samples were analysed using standard tube technique.
Titers of 1:64 or higher was considered as the high titre, for anti-A and anti B. Titers of ≤32 was
considered as low titre. Statistical analysis for the correlation between titre and donor age and
gender (Spearman’s correlation coefficient) was conducted using SPSS version 25.0.
Results: Overall, 45.5% of O donors had high antibody titres. Among them, the highest titres
were observed in age groups between 18-28 and 29-38. Antibody titre levels were considerably
low in older age groups (39-48 and 49-55). There was a statistically significant strong negative
correlation (r=-0.733, p<0.01) between donor age group with anti-A titre and with anti-B titre
(r=-0.756, p<0.01) among male donors. The above correlation in female donors was negative
[anti-A titre (r=-0.235, p=0.073) and anti-B titre (r=-0.331, p=0.01)], but not as strong as
observed in male donors.
Conclusions: Results show that only selected low titre O donor blood products can be used for
safe transfusions during non-specific ABO transfusions. It is recommended to do more studies
with higher number of samples to draw further conclusions.
