dc.contributor.author |
Sandamali, J. A. N. |
|
dc.contributor.author |
Hewawasam, R. P. |
|
dc.contributor.author |
Jayatilaka, K. A. P. W. |
|
dc.date.accessioned |
2022-09-13T08:58:44Z |
|
dc.date.available |
2022-09-13T08:58:44Z |
|
dc.date.issued |
2018-11-08 |
|
dc.identifier.citation |
Sandamali, J. A. N. , Hewawasam, R. P. , & Jayatilaka, K. A. P. W. (2018). Effect of Murraya koenigii Leaf Extract on Antioxidant Status, Lipid Peroxidation and Myeloperoxidase Activity in Wistar Rats Treated with Doxorubicin. 1 st Research Symposium of Faculty of Allied Health Sciences, University of Ruhuna, Galle, Sri Lanka, 10. |
en_US |
dc.identifier.issn |
2659-2029 |
|
dc.identifier.uri |
http://ir.lib.ruh.ac.lk/xmlui/handle/iruor/8304 |
|
dc.description.abstract |
Background: Doxorubicin is one of the most potent and widely used chemotherapeutic
agents. However, clinical utility is limited by dose-dependent cardiotoxicity, which leads to
severe heart failure. Previous studies favour reactive oxygen species as one of the main
factors responsible for doxorubicin-induced cardiotoxicity and administration of
antioxidants has been shown to protect cardiac tissues. Murraya koenigii leaves are rich in
various phytochemicals and have strong antioxidant potential.
Objectives: To investigate the effect of aqueous extract of Murraya leaf on antioxidant
status, lipid peroxidation and myeloperoxidase activity in Wistar rats treated with
doxorubicin.
Methodology: Wistar rats were divided into five groups of 10 animals in each. Group 1
served as the normal control. Group 2, plant extract control received 2.0 g/kg lyophilized
plant extract for 14 days and 10 mL/kg saline on 11th day. Group 3 received 2.0 g/kg
lyophilized plant extract for 14 days and 18 mg/kg doxorubicin on 11th day while group 4
received 10 mL/kg distilled water for 14 days, 18 mg/kg doxorubicin on 11th day. Group 5
which served as the positive control was given 10 mL/kg dH2O for 14 days, 180 mg/kg
dexrazoxane 0.5 hr prior to administration of doxorubicin (18 mg/kg). Animals were
sacrificed on the 15th day. A portion of heart tissues was collected for the estimation of
antioxidant parameters.
Results and conclusions: A significant reduction in reduced glutathione, glutathione
reductase, glutathione peroxidase, total antioxidant capacity, superoxide dismutase and
catalase activity and a significant increase in lipid peroxidation and myeloperoxidase
activity was observed in doxorubicin control compared to the normal control group
(p˂0.05). Plant extract treated group showed a significant decrease in lipid peroxidation,
myeloperoxidase activity and significant increase in rest of the parameters compared to the
doxorubicin control (p˂0.05). It can be concluded that Murraya leaf extract has the potential
to ameliorate doxorubicin induced oxidative stress and inflammation in Wistar rats. |
en_US |
dc.description.sponsorship |
Academic staff members of the Faculty of Allied Health Science, University of Ruhuna |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Faculty of Allied Health Sciences, University of Ruhuna, Galle, Sri Lanka |
en_US |
dc.subject |
Doxorubicin |
en_US |
dc.subject |
Murraya koenigii leaf extract |
en_US |
dc.subject |
oxidative stress |
en_US |
dc.subject |
Wistar rats |
en_US |
dc.title |
Effect of Murraya koenigii Leaf Extract on Antioxidant Status, Lipid Peroxidation and Myeloperoxidase Activity in Wistar Rats Treated with Doxorubicin |
en_US |
dc.type |
Presentation |
en_US |