Abstract:
Soil samples collected from abounded mines of Boryeong area in South Korea were used in isolating bacterial
strains and their capacity to solubilize inorganic phosphates and heavy metal tolerance were assessed in vitro.
Three different inorganic phosphate sources (Ca phosphate, Fe phosphate, and Al phosphate) and four
different heavy metals (Co, Cd, Pb and Zn) each with three concentrations (100 µg mL-1, 200 µg mL-1, and 400
µg mL-1) were used. The bacterial isolates PSB-1, PSB-2, PSB-3, and PSB-4 solubilized significantly higher
amount of Ca phosphate during the first five days of incubation though subsequent drop in soluble phosphorus
level in the medium was observed at the later stage (after 5 days) of the incubation. Solubilization of Ca
phosphate and Fe phosphate was concomitant with the acidification of the culture medium compared to the
control where it remained constant. Isolated strains could solubilize Fe phosphate to certain extent (25-45 µg
mL-1) though solubilization of Al phosphate was found negligible. All the isolates were tolerant to heavy
metals (Cd, Pb, and Zn) up to the concentration of 400 µg mL-1 except PSB-1 and PSB-8, which were shown
to be vulnerable to Co even at 100 µg mL-1. Heavy metal tolerant strains should be further evaluated for plant
growth promoting activities also under field conditions in order to assess their agricultural and environmental
significance