Control Effect of Root-knot Nematode (Meloidogyne incognita) by Biological Nematicide

Abstract

An nematophagous fungi Arthrobotrys thaumasia Nema-1 and Bacillus subtilis C-9, which degrade the collagen and gelatin, were isolated from horticulture plantation soil in Kyungpook Sungju-gun Seonnam-myun and Chungnam Gongju-gun Woosung-myun to develop biological nematode pesticide. When 5,000 mg kg-1 of A. thaumasia Nema-1 nematicide powder (7.0 ⨉ 103 cfu g-1) was treated to pot including Meloidogyne incognita, the number of nematode’s egg mass, which is a index of nematicidal activity, decreased to 35% compared to control. While the number of nematode’s egg mass decreased to 67% by treating the nematicide powder mixture of 5,000 mg kg-1 Nema-1 and B. subtilis C-9 (8.5 ⨉ 105 cfu g-1). Furthermore the number of nematode’s egg mass of the mixture containing cinnamon extract 10 mg kg-1, each 5,000 mg kg-1 of Nema-1 and C-9 nematicide powder was decreased to 84%, comparing to the result showed the number of nematode’s egg mass decreased to 24%, by the treatment of chemical nemato pesticide Fosthiazate 24 mg kg-1. These results suggested the mixture of microorganisms and plant extract was more effective biological nematicide than the case of only microorganism or plant extract for nematode control.