dc.contributor.author |
Fernando, Ganwarige Sumali N. |
|
dc.contributor.author |
Sergeeva, Natalia N. |
|
dc.contributor.author |
Frutos, Maria J. |
|
dc.contributor.author |
Boesch, Christine |
|
dc.date.accessioned |
2022-11-21T04:00:11Z |
|
dc.date.available |
2022-11-21T04:00:11Z |
|
dc.date.issued |
2022-08-15 |
|
dc.identifier.citation |
Fernando, G. S. N., Sergeeva, N. N., Frutos, M. J., Marshall, L. J., & Boesch, C. (2022). Novel approach for purification of major betalains using flash chromatography and comparison of radical scavenging and antioxidant activities. Food Chemistry, 385, 132632. |
en_US |
dc.identifier.issn |
0308-8146 |
|
dc.identifier.uri |
http://ir.lib.ruh.ac.lk/xmlui/handle/iruor/9405 |
|
dc.description.abstract |
The present study focused on the development of a new purification protocol suitable for betanin and other
major betalains, vulgaxanthin I, indicaxanthin and neobetanin, using flash chromatography which is a conve nient and fast method to isolate unstable materials. Following preliminary tests, a gradient procedure using
0–60% acetonitrile, with 0.1% (v/v) formic acid as mobile phase, was selected for the purification. Different
fractions were collected based on UV detection at 254 and 280 nm and purities were confirmed by reverse-phase
HPLC analysis to be 97%, 95%, 79% and 52% for betanin, indicaxanthin, vulgaxanthin I, and neobetanin,
respectively, with pigment yields ranging from 120 to 487 mg per 100 g of powdered raw material. Comparative
assessment of antioxidant and radial scavenging properties of individual betalains indicated highest potential for
betanin followed by neobetanin, vulgaxanthin I and indicaxanthin. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Elsevier |
en_US |
dc.subject |
Betalains |
en_US |
dc.subject |
purification |
en_US |
dc.subject |
flash chromatography |
en_US |
dc.subject |
HPLC |
en_US |
dc.subject |
antioxidant and radical scavegning activity |
en_US |
dc.title |
Novel approach for purification of major betalains using flash chromatography and comparison of radical scavenging and antioxidant activities |
en_US |
dc.type |
Article |
en_US |