Citation:Sun, P., Zhang, X., Zang, X., Zhou, X., Chen, Y., Arunakumara, K. K. I. U. & Liang, B., (2007). Anti-hypercalcemic effect of orally administered recombinant Saccharomyces cerevisiae expressing salmon calcitonin on hypercalcemic rats. Biotechnology letters, 29(7), 1013-1018. https://doi.org/10.1007/s10529-007-9352-3.
Date:2007
Abstract:
Oral delivery of salmon calcitonin
(sCT) to rats via a recombinant Saccharomyces
cerevisiae was assessed. A synthetic sCT gene was
cloned and expressed in S. cerevisiae yAGA2-
sCT. Recombinant salmon calcitonin (rsCT)
expression was detected by flow cytometry. The
resorption activity of osteoclasts was inhibited by
3 · 10–6 M rsCT. Oral administration of 5 g
lyophilized yAGA2-sCT/kg to hypercalcemic rats
decreased serum calcium from 2.8 ± 0.02–
2.7 ± 0.02 mM
