Abstract:
The viability of seeds can be determined with the use of 2,3,5-triphenyl tetrazolium chloride in a
shorter period of time. Tetrazolium staining is a dependable and accepted biochemical method for
testing the inherent germination of a seed lot. This is especially important in forest seeds which
take much longer time to germinate or may exhibit varying types of dormancy. To standardize the
preconditioning methods, soaking duration and concentration of the tetrazolium solution for
pungam (P. pinnata) seeds, an experiment was conducted at the laboratory with different methods
of precondition, soaking duration and concentration of the TZ solution.
