Short Communication In vitro Propagation of Vanda p te ris through Axillary Bud derived Protoeorm Culture.

dc.contributor.author	Begum, F.
dc.contributor.author	Islam, D.
dc.contributor.author	Paul, R.N.
dc.contributor.author	Mehedi, M.
dc.contributor.author	Mondal, S.R.
dc.date.accessioned	2023-05-04T03:53:21Z
dc.date.available	2023-05-04T03:53:21Z
dc.date.issued	2002-09-17
dc.identifier.issn	1391-3646
dc.identifier.uri	http://ir.lib.ruh.ac.lk/xmlui/handle/iruor/12552
dc.description.abstract	An efficient protocol for in vitro shoot multiplication of Vanda Pteris has been developed using axillary bud as explant. One axillary bud produced 4-6 protocorms on MS medium without any hormonal supplement. Subsequent propagation from protoeorm was performed on MS basal medium supplemented with different concentration and combination of BAP (03-3.0 mg/I) and NAA (0.0-2.0 mg/I). Maximum shoot multiplication and complete plant regeneration (Avg. 13.2 shoots/ protoeorm) was obtained in MS +2.0 mg/1 BAP+1.0 mg/1 NAA. Micropropagated shoots were easily rooted in Vz MS medium supplemented with 2.0 mg/! IBA. Addition of charcoal and ripe banana with boiled potato in the rooting media facilitated healthy roof production.	en_US
dc.language.iso	en	en_US
dc.publisher	Faculty of Agriculture, University of Ruhuna, SriLanka	en_US
dc.subject	Vanda Pteris	en_US
dc.subject	Axillary bud	en_US
dc.subject	Protoeorm	en_US
dc.subject	Shoot multiplication	en_US
dc.subject	Rooting	en_US
dc.title	Short Communication In vitro Propagation of Vanda p te ris through Axillary Bud derived Protoeorm Culture.	en_US
dc.type	Article	en_US