Abstract:
An efficient protocol for in vitro shoot multiplication of Vanda Pteris has been developed using axillary bud
as explant. One axillary bud produced 4-6 protocorms on MS medium without any hormonal supplement.
Subsequent propagation from protoeorm was performed on MS basal medium supplemented with
different concentration and combination of BAP (03-3.0 mg/I) and NAA (0.0-2.0 mg/I). Maximum shoot
multiplication and complete plant regeneration (Avg. 13.2 shoots/ protoeorm) was obtained in MS +2.0 mg/1
BAP+1.0 mg/1 NAA. Micropropagated shoots were easily rooted in Vz MS medium supplemented with 2.0
mg/! IBA. Addition of charcoal and ripe banana with boiled potato in the rooting media facilitated healthy
roof production.
