Abstract:
Seeds collected from 53 plants regenerated through direct somatic embryogenesis in Indian mustard
(Brassica juncea L.) Cv. Rai 5- from mesophyl! protoplasts were grown from P2 to P, to study the somaclonal
variation for different agronomic characters. Three lines showed 3-5 days early flowering while another
three lines showed late flowering compared to the control. Twelve lines selected for best yield in P2 were
evaluated for seed yield for seven generations (P3 to P,,). Most of the selections were comparable to the
control in seed yield. Seed oil content of protoclones in P3 and P4 was lower in comparison with the control.
