Comparative study of extraction on DNA using different protocols and from different body parts of a small Cyprinid fish species.

Abstract

Molecular studies play a considerable role in the modern world. In areas such as Medicine, Agriculture, Animal husbandry, and even Forensic studies. DNA is the fundamental material of the Molecular study. The first step of all Molecular studies is extraction of DNA. DNA is stored in the nucleus, mitochondria or cytoplasm in all living cells. The amount of DNA in the cells are vary with different organism. DNA could be extracted from different body parts of the organism. Drop of blood, hair, nails, skin, fins bones, and muscles are used for DNA extraction. Muscles are the commonly used body part for DNA extraction from fishes. But muscle biopsy is very harmful for fishes that it could lead to infection or affect the survival. This situation become worse when small fishes specially the endangered and endemic are concerned because it difficult to get a muscle sample without sacrificing the small fishes. Large numbers of DNA extraction protocols are used to extract DNA from different body parts of organisms. The aim of the present study was to determine the most suitable body part that exerts harm to the small fish species. To different protocols were compared using different body parts. In our study we observed that removal of a part of caudal fin is less harmful for' survival of fish. It is the fastest growing body part. In addition to the two different DNA extraction protocols were used in this study to compare the quality and quantity of DNA yielded using different body parts. According to our study using Phenol Chloroform method using without liquid Nitrogen yielded DNA with high purity. Phenol Chloroform method using with liquid Nitrogen yielded high concentrations of DNA.