Citation:Hewapathirana, T.D,Perera, T.C,Tudugala, R, Suresh, S,Kottahachchi, D.U.(2023).Prediction of Viable CD34 Count in Harvested Product/Peripheral Blood by Peripheral Blood Progenitor Count of Automated Haematology Analyzer in Multiple Myeloma Patients Undergoing Autologous Peripheral Blood Stem Cell Transplantation. International Research Symposium of the Faculty of Allied Health Sciences University of Ruhuna, Galle, Sri Lanka,31.
Date:2023-11-10
Abstract:
Background: Multiple myeloma (MM) is a clonal neoplastic disorder of plasma cells in the bone
marrow. Autologous Peripheral Blood Stem Cell Transplantation (PBSCT) is one of the promising
treatments of choice for MM. Success of PBSCT depends on the viable CD 34 cells (VCD34)
harvested from peripheral blood. Therefore, optimal engraftment can be assured by improved
estimation of CD 34 yield.
Objective: To assess the possibility of predicting VCD34 count in peripheral blood or harvested
product by apheresis of MM patients by using hematopoietic progenitor cell counts (HPC) in
peripheral blood (PB) from an automated hematology analyzer (AHA) compared to
flowcytometry
Methods: MM patients of age 40-65 years, admitted to Bone Marrow Transplant Unit of a tertiary
referral center were selected for the study (n=45). HPC in PB were enumerated using AHA and
compared with VCD34 count obtained by flowcytometry in both PB and apheresis. Statistical
analysis was performed using SPSS version 26.0. Data were separately tested for normalization,
followed by bivariate correlation analysis and linear regression analysis.
Results: HPC in PB obtained from AHA and VCD34 in both PB and apheresis obtained from
flowcytometry showed normal distribution. In the correlation bivariate analysis, HPC in PB
showed significant strong positive correlations with parameters; VCD34 by flowcytometry of PB
(r=0.942) and apheresis (r=0.778) (p<0.01)). Equations were derived using regression analysis;
VCD34 in PB by flowcytometry=1.210 (HPC of PB by AHA) -1.750 (R2=0.891) and VCD34 of
apheresis by flowcytometry=17.079 (HPC of PB by AHA) + 241.46 (R2=0.660).
Conclusions: HPC of PB by AHA can be used as a predictive marker to determine the VCD34
either in PB or apheresis as an alternative for flowcytometry. Further studies are required for the
validation of these findings for clinical application