Abstract:
Mikania micrantha has a diverse array of natural phytochemicals. The objective was to screen the clotting effect of dried leaves of M. micrantha to develop a topical herbal cream to arrest bleeding. The phytochemical screening revealed that the extract contains phenols, flavonoids, tannins, carbohydrates, saponins, terpenoids, and steroids. The clotting activity of the 70% ethanolic extract of M. micrantha was evaluated according to the standard Lee and White method. A concentration series of M. micrantha crude extract (5, 10, 15, and 20 mg/mL in dimethyl sulfoxide) was prepared for the assay, where tranexamic acid (10 mg/mL) was the positive control and dimethyl sulfoxide was the negative control. The highest coagulant activity was observed in the 20 mg/mL crude extract. The mean clotting time of the 20 mg/mL crude extract was 5.38 seconds (SD ± 8.89) and the mean clotting time of the positive control was 18.68 seconds (SD ± 13.51). So the leaves extract of M. micrantha exhibited significant clotting activity as compared to the positive control (p-value 0.018). Beeswax, liquid paraffin, borax, methyl paraben, distilled water, rose water, and 0.02 g of ethanol extract of M. micrantha was utilized to create a homogeneous, light green herbal cream. The slab technique was used to ensure homogeneous mixing of all ingredients. The cream had a pleasant odor, and its pH gradually decreased over two weeks. This study suggests that M. micrantha metabolites have coagulant properties. A new formulation with notable coagulant activity for treating topical bleeding was developed, with the potential for further commercial development.
