Abstract:
Three-dimensional structures of proteins are essential for understanding their
functions. X-ray crystallography and NMR spectroscopy are widely used
experimental methods for structure determination of proteins. However, there is
a large gap between the overwhelming number of available protein sequences
and experimentally determined protein structures. Homology modeling plays a
central role in fulfilling the above gap by determining protein structures. Here,
we modeled a three-dimensional structure of tRNA (guanine-N 1-)-
methyltransferase in Mycobacterium tuberculosis (tGNIMtase). It consists of
230 amino acids. The function of this protein is processing of the primary tRNA
transcript to yield a functional tRNA.