Detection of SSR and RAPD markers capable of distinguishing the genetic purity of tomato hybrid Hordi- Hybrid- 3

Abstract

Genetic purity is an important criterion for quality assurance of hybrid seed production. Grow- Out Test (GOT) is the usual practice to confirm hybrid purity based on morphological differences, which is time consuming, laborious, costly, and highly influenced by the environment. The main objective of the present study was to identify SSR and RAPD markers that can be potentially used for distinguishing purity of tomato hybrid HORDI-Hybrid-3. Twelve Simple Sequence Repeat (SSR) and fourteen Random Amplified Polymorphic DNA (RAPD) markers were used to confirm the genetic purity of tomato hybrid, HORDI- Hybrid-3. Hybrid seeds are produced by crossing two genetically distinct parental lines. During hybridization, genetic material from both parents is combined, resulting in offspring (hybrid seeds) that possess a mixture of alleles from each parent. Among the SSR markers tested, TOM 6 exhibited polymorphism, making it useful for distinguishing between the parental lines "V2" and "AVTO1130" of the tomato hybrid, HORDIHybrid- 3. Within the set of RAPD markers evaluated, both OPA 7 and J14 (PG12) displayed polymorphism between the parental lines, suggesting their potential utility in identifying the genetic purity of the hybrid variety HORDI-Hybrid-3. SSR marker TOM 6 can be verified to detect the genetic purity of the hybrid variety HORDI-Hybrid-3, after testing and confirming on a batch of randomly selected seeds of the hybrid variety HORDI-Hybrid-3 along with its parental line. The RAPD markers OPA7 and J14 (PG12), can be recommended to be used to develop Sequence Characterized Amplified Regions (SCAR) markers to detect the hybrid purity of variety HORDIHybrid- 3. These findings will aid in determining the genetic purity of tomato hybrid HORDI Hybrid 3 with greater precision in a shorter period.