Abstract:
Pink oyster mushroom is known as Pleurotus salmoneostramineus. This experiment was
undertaken to depict the favourable condition for mycelial growth, molecular identification and
phylogenetic relationship of the selected strains of pink oyster mushroom. Suitable temperature
and pH were obtained at 25ºC and 6, respectively for the mycelial growth of pink oyster
mushroom. Mushroom complete, glucose peptone and yeast malt extract culture media were the
favorable, while Hennerberg and Hoppkins were unfavorable. Dextrin was the best and xylose
was less effective carbon sources. Inorganic nitrogen sources were less effective for the mycelial
growth of P. salmoneostramineus. The sequences of internal transcribed spacer (ITS) region of
selected strains revealed that the total length ranged from 614 to 663 bp. The size of the ITS1
and ITS2 regions varied among the strains. Sequence analysis showed that 5.8S of rDNA
sequences were identical. Phylogenetic tree of the ITS region sequences indicated that strains of
P. salmoneostramineus belongs to a same cluster. The reciprocal homologies of the ITS region
sequences ranged from 98 to 100%. The strains of P. salmoneostramineus were also analyzed by
random amplification of polymorphic DNA (RAPD) with 20 arbitrary primers. RAPD results
suggested that tested strains of P. salmoneostramineus were genetically similar with some
variations.
