Abstract:
Radish (Raphanussativus L; Brassicaceae) is an important vegetable crop which produce an edible root with different
shapes and sizes. Apart from culinary purposes; radish has some medicinal properties as well. Major genetic
improvement of radish has been achieved by conventional plant breeding methods although it is a time and labour
consuming process. To overcome this situation, plant genetic engineering is used to improve novel plants with useful
agronomic characters. The success in plant genetic engineering is dependent on efficient tissue culture system with
high plant regeneration potential. This study was conducted to investigate the effects of different explants and
hormones on the plantlet regeneration of Radish (Raphanus sativus L; variety Beeralu Rabu). Seven different
hormone combinations on MS (Murashige and Skoog's) basal medium with 0.1mg/l NAA + 0 .1,0.5,1.0,1.5,2.0,2.5,3.0
mg/1 BAPas well as three types of explants: hypocotyl, leaves and root were employed. Completely Randomized
Design (CRD) with five replicates was used for the study Numbers of regenerated shoots were evaluated one month
after establishment. Anova (DMRT) test showed that there were significant effects at p<0.05 level on radish plantlets
regenerated with different treatments. Medium supplemented with 0.1mg/l NAA and 2.5mg/l BAP was the best
hormonal combination while hypocotyl explants (5 shoots/explant) was the best explant for the in vitro regeneration
of Radish variety Beeralu.
