Effects of Explants and Hormone Levels on Regeneration of Radish (Raphanus sativus L.) Variety Beeralu Rabu

Abstract

Radish (Raphanussativus L; Brassicaceae) is an important vegetable crop which produce an edible root with different shapes and sizes. Apart from culinary purposes; radish has some medicinal properties as well. Major genetic improvement of radish has been achieved by conventional plant breeding methods although it is a time and labour consuming process. To overcome this situation, plant genetic engineering is used to improve novel plants with useful agronomic characters. The success in plant genetic engineering is dependent on efficient tissue culture system with high plant regeneration potential. This study was conducted to investigate the effects of different explants and hormones on the plantlet regeneration of Radish (Raphanus sativus L; variety Beeralu Rabu). Seven different hormone combinations on MS (Murashige and Skoog's) basal medium with 0.1mg/l NAA + 0 .1,0.5,1.0,1.5,2.0,2.5,3.0 mg/1 BAPas well as three types of explants: hypocotyl, leaves and root were employed. Completely Randomized Design (CRD) with five replicates was used for the study Numbers of regenerated shoots were evaluated one month after establishment. Anova (DMRT) test showed that there were significant effects at p<0.05 level on radish plantlets regenerated with different treatments. Medium supplemented with 0.1mg/l NAA and 2.5mg/l BAP was the best hormonal combination while hypocotyl explants (5 shoots/explant) was the best explant for the in vitro regeneration of Radish variety Beeralu.