dc.contributor.author |
Amarasiri, S. S. |
|
dc.contributor.author |
Attanayake, A. P. |
|
dc.contributor.author |
Mudduwa, L. K. B. |
|
dc.contributor.author |
Jayatilaka, K. A. P. W. |
|
dc.date.accessioned |
2023-09-18T12:17:14Z |
|
dc.date.available |
2023-09-18T12:17:14Z |
|
dc.date.issued |
2022-12-15 |
|
dc.identifier.citation |
Amarasiri, S.S., Attanayake, A.P., Mudduwa, L.K.B. and Jayatilaka, K.A.P.W., 2022. Doxorubicin-induced nephrotoxicity model in Wistar rats: Characterization of biochemical parameters, histological and immunohistochemical assessment. Ceylon Journal of Science, 51(4), p.471-479.DOI: https://doi.org/10.4038/cjs.v51i4.8064 |
en_US |
dc.identifier.uri |
http://ir.lib.ruh.ac.lk/xmlui/handle/iruor/14730 |
|
dc.description.abstract |
This study characterizes the doxorubicin (DXR)-induced nephrotoxicity model in Wistar rats in terms of biochemical, histological, and immunohistochemical assessments, to provide a methodological reference model to investigate potential nephroprotective therapeutics. The experiments were carried out using four groups of healthy male Wistar rats (six rats per group) administered with a single intraperitoneal dose of normal saline (vehicle group) and DXR at 17, 20, and 23 mg/kg doses, respectively. The rats were sacrificed under anaesthesia on the seventh day after DXR administration and blood, urine, and kidney tissues were collected for investigations. The DXR dose of 17 mg/kg showed mild changes in biochemical parameters, whereas 23 mg/kg resulted in increased mortality (33%). Serum concentrations of creatinine, urea nitrogen, β2-microglobulin, urine concentrations of total protein, creatinine, and urea increased with the increased dose of DXR while serum concentrations of total protein and albumin decreased (p<0.05). Assessment of histopathology revealed the features of acute tubular injury and immunohistochemical studies revealed increased apoptosis and inflammatory changes. Based on the findings, the DXR dose of 20 mg/kg was selected as the optimal dose for the establishment of an acute nephrotoxicity model in Wistar rats to explore potential nephroprotective drug leads. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Ceylon Journal of Science |
en_US |
dc.subject |
Doxorubicin-induced acute tubular injury |
en_US |
dc.subject |
Apoptosis |
en_US |
dc.subject |
Histopathology |
en_US |
dc.subject |
Immunohistochemistry |
en_US |
dc.subject |
Inflammation |
en_US |
dc.title |
Doxorubicin-induced nephrotoxicity model in Wistar rats: Characterization of biochemical parameters, histological and immunohistochemical assessment |
en_US |
dc.type |
Article |
en_US |