Abstract:
The Philodendron Black Cardinal plant is an ornamental plant with a higher market
demand. However, despite its demand the production is low, and there is no any exact
surface sterilization protocol available for the preparing of the In-vitro propagation.
Therefore, this investigation was aimed at developing a surface sterilization protocol
to select a suitable multiplication media. The experiment was conducted at TC
PLANT, Homagama. For the surface sterilization protocol, mother plants were
treated with fungicide and then shoot tips were taken. Shoot tips were washed in
Tepol, soaked in fungicide, Clorox solution [(10%, 15%, 20%, and 30%
concentration) for a time duration of 20 and 30-minutes, and 70% alcohol, Mercuric
chloride (HgCl2) were inoculated to the media. During the initiation period,
contamination rate, implementation of the length in shoots and viability of shoots
were taken as data. The experiment was led in a Complete Randomized Design
(CRD) and all the taken data were analyzed with SAS software package. According
to the data analysis 15%, (30 minutes) Clorox concentration was selected as the best
surface sterilization protocol. In the multiplication stage, 6-Benzylaminopurine
(BAP) levels were changed in the Murashige and Skoog (MS) medium. Data obtained
for one month were used to select the most suitable medium for multiplication.
Medium with the BAP 5g/L was selected as the best multiplication media based on
the number of shoots obtained.
