Abstract:
Genetic purity is an important criterion for quality assurance of hybrid seed production. Grow-
Out Test (GOT) is the usual practice to confirm hybrid purity based on morphological differences,
which is time consuming, laborious, costly, and highly influenced by the environment. The main
objective of the present study was to identify SSR and RAPD markers that can be potentially used
for distinguishing purity of tomato hybrid HORDI-Hybrid-3. Twelve Simple Sequence Repeat
(SSR) and fourteen Random Amplified Polymorphic DNA (RAPD) markers were used to confirm
the genetic purity of tomato hybrid, HORDI- Hybrid-3. Hybrid seeds are produced by crossing two
genetically distinct parental lines. During hybridization, genetic material from both parents is
combined, resulting in offspring (hybrid seeds) that possess a mixture of alleles from each parent.
Among the SSR markers tested, TOM 6 exhibited polymorphism, making it useful for
distinguishing between the parental lines "V2" and "AVTO1130" of the tomato hybrid, HORDIHybrid-
3. Within the set of RAPD markers evaluated, both OPA 7 and J14 (PG12) displayed
polymorphism between the parental lines, suggesting their potential utility in identifying the
genetic purity of the hybrid variety HORDI-Hybrid-3. SSR marker TOM 6 can be verified to detect
the genetic purity of the hybrid variety HORDI-Hybrid-3, after testing and confirming on a batch
of randomly selected seeds of the hybrid variety HORDI-Hybrid-3 along with its parental line. The
RAPD markers OPA7 and J14 (PG12), can be recommended to be used to develop Sequence
Characterized Amplified Regions (SCAR) markers to detect the hybrid purity of variety HORDIHybrid-
3. These findings will aid in determining the genetic purity of tomato hybrid HORDI
Hybrid 3 with greater precision in a shorter period.
