Abstract:
Vibrio nigripulchritudo causes vibriosis in penaeid shrimps. Here, we used Illumina and
Nanopore sequencing technologies to sequence the genomes of three of its strains
(TUMSAT-V.
nig1, TUMSAT-V.
nig2, and TUMSAT-V.
nig3) to explore opportunities for
disease management. Putative virulence factors and mobile genetic elements were
detected while evaluating the phylogenetic relationship of each isolated strain. The
genomes consisted of two circular chromosomes (I and II) plus one or two plasmids.
The size of chromosome I ranged from 4.02 to 4.07 Mb with an average GC content
of 46%, while the number of predicted CDSs ranged from 3563 to 3644. The size of
chromosome II ranged from 2.16 to 2.18 Mb, with an average GC content of 45.5%,
and the number of predicted CDSs ranged from 1970 to 1987. Numerous virulence
genes were identified related to adherence, antiphagocytosis, chemotaxis, motility,
iron uptake, quorum sensing, secretion systems, and toxins in all three genomes.
Higher numbers of prophages and genomic islands found in TUMSAT-V.
nig1 suggest
that the strain has experienced numerous horizontal gene transfer events. The
presence of antimicrobial resistance genes suggests that the strains have multidrug
resistance. Comparative genomic analysis showed that all three strains belonged to
the same clade.
