Nephroprotective activity of Vetiveria zizanioides (L.) Nash supplement in doxorubicin-induced nephrotoxicity model of Wistar rats. Journal of Food Biochemistry

Abstract

The nephroprotective effect of standardized aqueous root extract of Vetiveria ziza nioides (L.) Nash (Family: Poaceae) was investigated in doxorubicin-induced (20 mg/ kg, ip) experimental nephrotoxicity model of Wistar rats. The freeze-dried aqueous refluxed (4 hr) root extract of V. zizanioides (25, 50; equivalent human therapeutic dose and 100 mg/kg) was administered separately to nephrotoxic Wistar rats (n = 6/ group). Supplement of V. zizanioides resulted a dose-dependent reduction in raised serum creatinine, β2-microglobulin, and blood urea nitrogen and a subsequent in crease in serum total protein and albumin in nephrotoxic rats (p < .05). An attenua tion of the doxorubicin-induced features of renal parenchymal injury was observed on H- and E-stained sections of the kidney tissues. Nootkatone, dehydroaromaden drene, isokhusenic acid, α-vetivone, and isolongifolene were identified in the metha nol extract of V. zizanioides based on the GC-MS chromatogram analysis. The findings revealed that the supplement of standardized aqueous root extract of V. zizanioides had a significant dose-dependent nephroprotective activity against doxorubicin induced experimental nephrotoxicity